Word Count: 2500w+250w ref
Format: Case Study
You are to write a case study review consists of approximately 2500 words (excluding references).
A cholesterol metabolite, 3β,7α-dihydroxycholest-5-en-26-oic (3β,7α-diHCA) has recently been shown to promote motor neuron survival. In order to understand the mechanism behind the activity of 3β,7α-diHCA, could you design an experimental strategy to quantify the protein expression affected by 3β,7α-diHCA.
This should include:
1, experiment design
2, sample preparation, how are protein extracted from tissue
3, method for protein quantification
4, mass spectrometry analysis, what the information obtained by perform MS and MS/MS analysis
5, database search, how dose the software match spectrum with protein database and perform quantification